Activity-based PRMT7 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
PRMT7
Enzyme
Human PRMT7
Synonym
Protein arginine N-methyltransferase 7, Histone-arginine N-methyltransferase PRMT7, KIAA1933
Substrates
GST-GAR, 5 µM
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 340 |
| Chaetocin | 144,000 |
| Sinefungin | 550 |
| Suramin | >200,000 |
Scientific Information
Q9NVM4 (http://www.uniprot.org/uniprot/Q9NVM4)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for PRMT7
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