Full-length PTPN11/SHP2 (E76K) Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PTPN11/SHP2 (E76K)-FL
Enzyme
Human Full-length PTPN11/SHP2 (E76K)
Synonyms
Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, PTP2C, SHPTP2
Substrates
Fluorogenic substrate DiFMUP, 100 μM
With/without activating peptide [H2N-LN(pY)IDLDLV(dPEG8)LST(pY)ASINFQK-amide]
Assay Properties
Reaction
Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SHP099 (without peptide) | 2,600 |
| SHP099 (with peptide) | >100,000 |
Scientific Information
Q06124 (http://www.uniprot.org/uniprot/Q06124)
Screening Location
Malvern, USA
Reference Compound IC50 for PTPN11/SHP2 (E76K)-FL
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