PTPN1/PTP1B-CD Phosphatase Assay Service

PTPN1/PTP1B Catalytic Domain Phosphatase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.

Target Overview

CAT#

PTPN1/PTP1B-CD

Enzyme

Human PTPN1/ PTP1B (Catalytic Domain)

Synonyms

Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B

Substrates

Fluorogenic substrate DiFMUP, 2 μM

Assay Properties

Reaction

Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
PTP1B Inhibitor 3,432
PHPS1 430
NSC-87877 5,805

Scientific Information

P18031 (http://www.uniprot.org/uniprot/P18031)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Phosphatase Assay Services for Drug Discovery

Reference Compound IC50 for PTPN1/PTP1B-CD

Reference Compound IC50 for PTPN1/PTP1B-CD