Activity-based PYK2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
PYK2
Enzyme
Human PYK2, wt
HGNC
PTK2B
Manning ID
PYK2
Synonym
CADTK, CAKB, FADK2, FAK2, PKB, PTK, PYK2, RAFTK, CAK beta, PTK2B, CAKB, FADK2
UniProt
Q14289 (FAK2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 11 |
| Sorafenib | 9800 |
| Sunitinb | 670 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | PYK2 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – PYK2 |
| Product Number |
0363-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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