Activity-based RAF1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RAF1
Enzyme
Human RAF1
HGNC
RAF1
Synonym
c-RAF
Substrates
MEK1 (K97R), 1 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| GW 5074 | 6.7 |
Scientific Information
P04049 (http://www.uniprot.org/uniprot/P04049)
Screening Location
Malvern, USA
Reference compound IC50 for RAF1
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