Activity-based RAF1 (R391W) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RAF1 (R391W)
Enzyme
Human RAF1 (R391W)
HGNC
RAF1 (R391W)
Synonym
RAF proto-oncogene serine/threonine-protein kinase, Raf-1, C-RAF, cRaf, RAF, c-RAF
Substrates
MEK1 (K97R), 5 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | >100,000 |
| Ro 31-8220 | >100,000 |
| GW 5074 | 5.4 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
P04049 (http://www.uniprot.org/uniprot/P04049)
Screening Location
Malvern, USA
Reference compound IC50 for RAF1 (R391W)
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