Activity-based RAF1 YDYD kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
RAF1 YDYD
Enzyme
Human RAF1 YDYD, wt
HGNC
RAF1 (Y340/Y341D)
Manning ID
RAF1
Synonym
cRaf, RAF1, c-RAF, RAF1 Y340D/Y341D (untagged), RAF1 YDYD
UniProt
P04049 (RAF1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
MEK1-K97M, ATP
Reaction
MEK1-K97M + 33P-ATP = 33P-MEK1-K97M + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Sorafenib | 120 |
| Sunitinib | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | RAF1 YDYD |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – RAF1 YDYD (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0792-0000-2 |
| Web Shop | Reaction Biology Online Shop |
PDF