rDHODH Dehydrogenase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
rDHODH
Enzyme
Rat DHODH
Synonym
Dihydroorotate dehydrogenase (quinone) mitochondrial, DHOdehase, Dihydroorotate oxidase
Scientific Information
Assay Properties
Substrate
25 µM L-DHO, 50 µM resazurin, Cofactor FMN
Reaction
L-DHO + FMN = Orotate + FMNH2
FMNH2 + Resazurin = FMN + Resorufin
Resorufin fluorescence, End point measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| BAY-2402234 | 1500 |
| Brequinar | 375.7 |
| Teriflunomide | 5.3 |
| Leflunomide | 104.3 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Nucleotide Metabolism Pathway Assays
Reference compound inhibition of rDHODH
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