RET-CCDC6 (PTC1) Kinase Assay Service

Activity-based RET-CCDC6 (PTC1) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

-RET, CCDC6 (PTC1)

Enzyme

Human CCDC6-RET (PTC1) fusion

HGNC

RET-CCDC6

Synonym

CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Substrates

Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 5.1
PP2 28
AG1478 450
EGFR/ErbB Inhibitor >100,000
D-64406 140

Scientific Information

CCDC6; Q16204 (http://www.uniprot.org/uniprot/Q16204)
RET; P07949 (http://www.uniprot.org/uniprot/P07949)

Screening Location

Malvern, USA

Reference compound IC50 for RET-CCDC6 (PTC1)

PDF