Activity-based RET kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
RET
Enzyme
Human RET, wt
HGNC
RET
Manning ID
RET
Synonym
PTC, MTC1, HSCR1, MEN2A, MEN2B, RET51, CDHF12, RET, CDHR16, RET-ELE1, RET wt
UniProt
P07949 (RET_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY+ 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 20 |
| Sorafenib | 53 |
| Sunitinib | 41 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | RET |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 RET Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – RET (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1090-0000-1 |
| Web Shop | Reaction Biology Online Shop |