Activity-based RET R749T kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
RET R749T
Enzyme
Human RET R749T, mutant
HGNC
RET (R749T)
Manning ID
RET
Synonym
RET, CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51, RET-ELE1, RET R749T, RET (R749T)
UniProt
P07949 (RET_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY+ 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 22 |
| Sorafenib | 23 |
| Sunitinib | 49 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | RET R749T |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 RET Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – RET R749T (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1092-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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