RET (V804L)-KIF5B Kinase Assay Service

Activity-based RET (V804L)-KIF5B kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

RET (V804L), KIF5B-

Enzyme

Human KIF5B-RET (V804L) Fusion protein

HGNC

RET (V804L)-KIF5B

Synonym

CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Scientific Information

KIF5B: P33176 (http://www.uniprot.org/uniprot/P33176)
RET: P07949 (http://www.uniprot.org/uniprot/P07949)

Assay Properties

Substrates

Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.7
PP2 >100,000
AG1478 >100,000
EGFR/Erb Inhibitor >100,000
D-64406 350

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for RET (V804L)-KIF5B

Reference compound IC50 for RET (V804L)-KIF5B

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