Activity-based RET (V804L) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RET (V804L)
Enzyme
Human RET (V804L)
HGNC
RET (V804L)
Synonym
CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51
Scientific Information
P07949 (http://www.uniprot.org/uniprot/P07949)
Assay Properties
Substrates
Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 2.2 |
| PP2 | >100,000 |
| AG1478 | >100,000 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 540 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for RET (V804L)
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