Activity-based ROCK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ROCK1
Enzyme
Human ROCK1
HGNC
ROCK1
Synonym
P160ROCK
Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.93 |
| Ro 31-8220 | 3,700 |
| GW 5074 | 11,500 |
| H-89 | 230 |
Scientific Information
Q13464 (http://www.uniprot.org/uniprot/Q13464)
Screening Location
Malvern, USA
Reference compound IC50 for ROCK1
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