ROCK1 Kinase Assay Service

Activity-based ROCK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
ROCK1

Enzyme
Human ROCK1

HGNC
ROCK1

Synonym
P160ROCK

Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.93
Ro 31-8220 3,700
GW 5074 11,500
H-89 230
Scientific Information
Q13464 (http://www.uniprot.org/uniprot/Q13464)

Screening Location
Malvern, USA

Reference compound IC50 for ROCK1
Reference compound IC50 for ROCK1