Activity-based ROS1 (G2101C) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ROS1 (G2101C)
Enzyme
Human ROS1 (G2101C)
HGNC
ROS1 (G2101C)
Synonym
Proto-oncogene tyrosine-protein kinase ROS, Receptor tyrosine kinase c-ros oncogene 1, MCF3
Substrates
Peptide substrate, [KKKSPGEYVNIEFG], 20 µM
ATP 1 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.1 |
| PP2 | 4,400 |
| AG1478 | 1,500 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 9,900 |
Scientific Information
P08922 (http://www.uniprot.org/uniprot/P08922)
Screening Location
Malvern, USA
Reference compound IC50 for ROS1 (G2101C)
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