Activity-based ROS1 (L2086F) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ROS1 (L2086F)
Enzyme
Human ROS1 (L2086F)
HGNC
ROS1 (L2086F)
Synonym
Proto-oncogene tyrosine-protein kinase ROS, Receptor tyrosine kinase c-ros oncogene 1, MCF3
Scientific Information
P08922 (http://www.uniprot.org/uniprot/P08922)
Assay Properties
Substrates
Peptide substrate, [KKKSPGEYVNIEFG], 20 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.6 |
| PP2 | 1,100 |
| AG1478 | 180 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 20,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for ROS1 (L2086F)
