Activity-based ROS/ROS1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ROS/ROS1
Enzyme
Human ROS/ROS1
HGNC
ROS1
Synonym
ROS, MCF3
Substrates
Peptide substrate, [KKKSPGEYVNIEFG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.1 |
| PP2 | 3,100 |
| AG1478 | 2,600 |
| PDGFR I-III | >20,000 |
| D-64406 | >20,000 |
Scientific Information
P08922 (http://www.uniprot.org/uniprot/P08922)
Screening Location
Malvern, USA
Reference compound IC50 for ROS/ROS1
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