Activity-based RPS6KA2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
RPS6KA2
Enzyme
Human RPS6KA2, wt
HGNC
RPS6KA2
Manning ID
RSK1
Synonym
HU-2, MAPKAPK1C, RSK, RSK3, RPS6KA2, RSK1
UniProt
Q15349 (KS6A2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
AGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
bio-4xCHKT, ATP
Reaction
bio-4xCHKT + 33P-ATP = 33P-bio-4xCHKT + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3 |
| Sorafenib | 4000 |
| Sunitinib | 800 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | RPS6KA2 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
PDF