RSK1 Kinase Assay Service - Reaction Biology

Activity-based RSK1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

RSK1

Enzyme

Human RSK1

HGNC

RPS6KA1

Synonym

RSK1, HU-1, MAPKAPK1, p90Rsk

Substrates

Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q15418 (http://www.uniprot.org/uniprot/Q15418)

Screening Location

Malvern, USA

Reference compound IC50 for RSK1