Activity-based RSK2 (I416V) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RSK2 (I416V)
Enzyme
Human RSK2 (I416V)
HGNC
RPS6KA3 (I416V)
Synonym
RSK2, HU-3, MRX19, CLS
Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.1 |
| Ro 31-8220 | <0.2 |
| GW5074 | 7,400 |
| H-89 | 310 |
| Rottlerin | 18,000 |
Scientific Information
P51812 (http://www.uniprot.org/uniprot/P51812)
Screening Location
Malvern, USA
Reference compound IC50 for RSK2 (I416V)
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