RSK2 Kinase Assay Service - Reaction Biology

Activity-based RSK2 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

RSK2

Enzyme

Human RSK2

HGNC

RPS6KA3

Synonym

RSK2, HU-3, MRX19, CLS

Substrates

Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

P51812 (http://www.uniprot.org/uniprot/P51812)

Screening Location

Malvern, USA

Reference compound IC50 for RSK2