RSK3 Kinase Assay Service

Activity-based RSK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
RSK3

Enzyme
Human RSK3

HGNC
RPS6KA2

Synonym
RSK3, HU-2

Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.5
Ro 31-8220 <0.5
GW 5074 710
H-89 1,000
Scientific Information
Q15349 (http://www.uniprot.org/uniprot/Q15349)

Screening Location
Malvern, USA

Reference compound IC50 for RSK3
Reference compound IC50 for RSK3