RSK4 Kinase Assay Service

Activity-based RSK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
RSK4

Enzyme
Human RSK4

HGNC
RPS6KA6

Synonym
RSK4

Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.1
Ro 31-8220 <0.1
GW5074 990
H-89 1,100
Rottlerin >20,000
Scientific Information
Q9UK32 (http://www.uniprot.org/uniprot/Q9UK32)

Screening Location
Malvern, USA

Reference compound IC50 for RSK4
Reference compound IC50 for RSK4