Activity-based RSK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RSK4
Enzyme
Human RSK4
HGNC
RPS6KA6
Synonym
RSK4
Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.1 |
| Ro 31-8220 | <0.1 |
| GW5074 | 990 |
| H-89 | 1,100 |
| Rottlerin | >20,000 |
Scientific Information
Q9UK32 (http://www.uniprot.org/uniprot/Q9UK32)
Screening Location
Malvern, USA
Reference compound IC50 for RSK4
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