Activity-based SAK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
SAK
Enzyme
Human SAK, wt
HGNC
PLK4
Manning ID
PLK4
Synonym
SAK, STK18, PLK4, PLK4/SAK
UniProt
O00444 (PLK4_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other (CAMK)
Assay Properties
Assay Format
33PanQinaseTM
Substrates
p38-alpha KA, ATP
Reaction
p38-alpha KA + 33P-ATP = 33P-p38-alpha KA + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 5 |
| Sorafenib | 1400 |
| Sunitinib | 870 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | SAK |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – SAK (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0306-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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