SARS-CoV-2 Mpro Protease Assay (3CL Protease Assay) for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
Mpro
Enzyme
SARS-CoV-2 Mpro
Synonym
3CL Protease, SARS-CoV-2 Main Protease, SARS-CoV-2 3C-like Proteinase, 3CLpro
Substrates
Fluorogenic substrate [NH2-C(EDANS)VNSTQSGLRK(DABCYL)M-COOH], 5 μM
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| Presence of 1 mM DTT | Compounds | IC50 (nM) |
|---|---|---|
| GC376 | 6.5 | |
| Carmofur | 36,000 | |
| Ebselen | >100,000 | |
| Absence of DTT | Compounds | IC50 (nM) |
| GC376 | 5.5 | |
| Carmofur | 35 | |
| Ebselen | 25 |
Scientific Information
Accession #QHD43415.1
Screening Location
Malvern, USA
Further information
More information can be found on our website Protease Assay Services.
Reference compound IC50 for SARS-CoV-2 Mpro
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