SARS-CoV-2 PLpro Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
PLpro
Enzyme
SARS-CoV-2 PLpro
Synonym
SARS-CoV-2 Papain-like Protease, NS3
Substrates
Fluorogenic substrate [Z-RLRGG-AMC], 10 μM or [Ubiquitin-AMC], 100 nM
Assay Properties
Readout
Enzymatic activity
Reference Compound IC50s
| [Z-RLRGG-AMC] | Compounds | IC50 (μM) |
|---|---|---|
| PLpro Inhibitor | 0.92 | |
| UCHL1 I | 253 | |
| UIPase I | 14.8 | |
| UCHL3 I | 14.2 | |
| [Ubiquitin-AMC] | Compounds | IC50 (μM) |
| PLpro Inhibitor | 0.98 | |
| UCHL1 I | 27.5 | |
| UIPase I | 15.6 | |
| UCHL3 I | 8.7 |
Scientific Information
Accession #YP_009725299.1
Screening Location
Malvern, USA
Further information
More information can be found on our website Protease Assay Services.
Reference compound IC50 for SARS-CoV-2 PLpro
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