SENP2 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
SENP2
Enzyme
Human SENP2
Synonym
Sentrin-specific protease 2, Sentrin/SUMO-specific protease SENP2, SMT3-specific isopeptidase 2, Axam2, Smt3ip2
Substrate
Fluorogenic substrate [SUMO1-AMC], 300 nM
Assay Properties
Reaction
Cleavage of SUMO1 for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
Compounds IC50 (µM)
| UCHL3 I | 2.8 |
|---|
Scientific Information
Q9HC62 (http://www.uniprot.org/uniprot/Q9HC62)
Screening Location
Malvern, USA
Reference compound IC50 for SENP2
PDF