SERT (serotonin transporter) Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
SERT Binding
GPCR Target
Human SERT
Source
Membrane preparation from HEK293 cells stably transfected with human SERT receptor
Scientific Information
SC6A4 (https://www.uniprot.org/uniprotkb/P31645/entry)
Assay Properties
Protocol
Radioligand filter binding to SERT membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| Imipramine (55.9) | Imipramine (20) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for SERT
PDF