SET1B Methyltransferase Assay Service

Activity-based SET1B Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.

Target Overview

CAT#

SET1B

Enzyme

Human SET1B

Synonym

Complex of SET1B: Histone-lysine N-methyltransferase SETD1B, Lysine N-methyltransferase 2G, KIAA1076, KMT2G
WDR5: WD repeat-containing protein 5, BMP2-induced 3-kb gene protein
RBBP5: Retinoblastoma-binding protein 5, Retinoblastoma-binding protein RBQ-3
ASH2L: Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein
DPY30: Protein dpy-30 homolog, Dpy-30-like protein

Substrates

Core Histone, 0.05 mg/ml
SAM, 1 µM

Assay Properties

Reaction

S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SAH 3,200
Sinefugin 4,400
BIX01294 >100,000
Chaetocin 210

Scientific Information

SET1B Q9UPS6 (http://www.uniprot.org/uniprot/Q9UPS6)
WDR5 P61964 (http://www.uniprot.org/uniprot/P61964)
RBBP5 Q15291 (http://www.uniprot.org/uniprot/Q15291)
ASH2L Q9UBL3 (http://www.uniprot.org/uniprot/Q9UBL3)
DPY30 Q9C005 (http://www.uniprot.org/uniprot/Q9C005)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Methyltransferase Assays.

Reference compound IC50 for SET1B

Reference compound IC50 for SET1B

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