SET7/9 Methyltransferase Assay Service

Activity-based SET7/9 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.

Target Overview

CAT#

SET7/9

Enzyme

Human SET7/9

Synonym

Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, SET domain-containing protein 7, SET7/9, Lysine N-methyltransferase 7, KIAA1717, KMT7, SET7, SET9

Substrates

Core Histone, 0.05 mg/ml
SAM, 1 µM

Assay Properties

Reaction

S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SAH 84,400
Sinefugin 5,200

Scientific Information

Q8WTS6 (http://www.uniprot.org/uniprot/Q8WTS6)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Methyltransferase Assays.

Reference compound IC50 for SET7/9

Reference compound IC50 for SET7/9