Activity-based SGK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SGK2
Enzyme
Human SGK2
HGNC
SGK2
Synonym
H-SGK2, dJ138B7.2
Substrates
Peptide substrate, [KGSGSGRPRTSSFAEG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 25.7 |
| Ro 31-8220 | 310 |
| GW 5074 | 2,600 |
| H-89 | 2,600 |
Scientific Information
Q9HBY8 (http://www.uniprot.org/uniprot/Q9HBY8)
Screening Location
Malvern, USA
Reference compound IC50 for SGK2
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