Activity-based SGK3/SGKL kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SGK3
Enzyme
Human SGK3
HGNC
SGK3
Synonym
CISK, SGK2, SGKL
Substrates
Peptide substrate, [GRPRTSSFAEG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 236 |
| Ro 31-8220 | 745 |
| Rottlerin | 2,300 |
Scientific Information
Q96BR1 (http://www.uniprot.org/uniprot/Q96BR1)
Screening Location
Malvern, USA
Reference compound IC50 for SGK3/SGKL
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