Activity-based SIK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SIK2
Enzyme
Human SIK2
HGNC
SIK2
Synonym
QIK, SNF1LK2
Substrates
Peptide substrate, [AMARAASAAALARRR], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.1 |
| Ro 31-8220 | 1,400 |
| GW5074 | 8,300 |
| H-89 | 11,000 |
| Rottlerin | >20,000 |
Scientific Information
Q9H0K1 (http://www.uniprot.org/uniprot/Q9H0K1)
Screening Location
Malvern, USA
Reference compound IC50 for SIK2
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