SIRT3 Sirtuin Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
SIRT3
Enzyme
Human SIRT3 (NAD+-dependent enzyme)
Synonyms
NAD-dependent deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3, SIR2L3, Class III HDAC
Assay Properties
Readout
Enzymatic activity
Substrates
Fluorogenic peptide from p53 residues 379-382 (RHKKAc), 50 μM
NAD+, 500 μM
Reference Compound IC50s
| Compounds | IC50 (μM) |
|---|---|
| Nicotinamide | 35.1 |
Scientific Information
Q9NTG7 (http://www.uniprot.org/uniprot/Q9NTG7)
Screening Location
Malvern, USA
Reference Compound IC50 for SIRT3
PDF