SIRT5 Sirtuin Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
SIRT5
Target Overview
Enzyme
Human SIRT5 (NAD+-dependent enzyme)
Synonyms
NAD-dependent protein deacylase sirtuin-5, Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIR2L5, Class III HDAC
Assay Properties
Readout
Enzymatic activity
Substrates
Fluorogenic peptide (Ac-Lys-succ-AMC), 50 μM
NAD+, 500 μM
Reference Compound IC50s
| Compounds | IC50 (μM) |
|---|---|
| Nicotinamide | 24.2 |
Scientific Information
Q9NXA8 (http://www.uniprot.org/uniprot/Q9NXA8)
Screening Location
Malvern, USA
Reference Compound IC50 for SIRT5
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