Activity-based SLK/STK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SLK
Enzyme
Human SLK
HGNC
SLK
Synonym
KIAA0204, STK2
Substrates
Histone H3, 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 3.9 |
| Ro 31-8220 | 38.9 |
| GW5074 | 18,000 |
| H-89 | 42,000 |
Scientific Information
Q9H2G2 (http://www.uniprot.org/uniprot/Q9H2G2)
Screening Location
Malvern, USA
Reference compound IC50 for SLK/STK2
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