SMARCA2b Bromodomain SPR Assay Service

To test the direct binding to the SMARCA2b bromodomain using surface plasmon resonance (SPR) technique

Target Overview

Target

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 (bromodomain)

HGNC symbol

SMARCA2

Synonyms

BAF190B, BRM, SNF2L2

Uniprot page

P51531 (https://www.uniprot.org/uniprot/P51531)

Screening location

Malvern, PA, USA

Further information

More information can be found on our website SPR Assay Services for Drug Discovery

Binding kinetics of reference compound (PFI-3) to the bromodomain of SMARCA2b.

SMARCA2b SPR

The data was collected on a Biacore 8K using multi-cycle kinetics. A reduced DMSO condition was used. The data was analyzed using both a 1:1 kinetic binding model and a steady state affinity model to determine binding affinity (KD).