SMYD2 Methyltransferase Assay Service

Activity-based SMYD2 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.

Target Overview

CAT#

SMYD2

Enzyme

Human SMYD2

Synonym

SET and MYND domain-containing protein 2, HSKM-B Lysine N-methyltransferase 3C, KMT3C

Substrates

Histone H3, 5 µM
SAM, 1 µM

Assay Properties

Reaction

S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SAH 526
Sinefugin 15,000

Scientific Information

Q9NRG4 (http://www.uniprot.org/uniprot/Q9NRG4)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Methyltransferase Assays.

Reference compound IC50 for SMYD2

Reference compound IC50 for SMYD2