Activity-based SNARK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
SNARK
Enzyme
Human SNARK, wt
HGNC
NUAK2
Manning ID
NuaK2
Synonym
DKFZp434J037 (predicted protein only!), NUAK2, DKFZp434J037, SNARK, SNARK/NUAK2
UniProt
Q9H093 (NUAK2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CAMK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Histon H2B, ATP
Reaction
Histon H2B + 33P-ATP = 33P-Histon H2B + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 5 |
| Sorafenib | 10000 |
| Sunitinib | 4000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | SNARK |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – SNARK (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0414-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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