SP100 Bromodomain Assay Service (ThermoShift)

SP100-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview

CAT#

Tm-SP100-GST

Protein

Human SP100 bromodomain, GST-tagged

Synonyms

Nuclear dot-associated Sp100 protein

Assay Properties

Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (2.08 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

47,5

Reference Compound ΔTms:

RVX-208 – 1 (Purple)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, GSK2801 and PFI3)

Scientific Information

P23497 (http://www.uniprot.org/uniprot/P23497)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak