SP140-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-SP140-H
Protein
Human SP140 bromodomain, His-tagged
Synonyms
Lymphoid-restricted homolog of Sp100; Nuclear autoantigen Sp-140; Speckled 140 kDa
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (4.2 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
47
Reference Compound ΔTms:
PFI1 – 2 (Grey)CBP112 – 2 (Purple)Bromosporine – 1.5 (Blue)No stabilization was observed in the presence of other control compounds (JQ1, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)
Scientific Information
Q13342 (http://www.uniprot.org/uniprot/Q13342)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
