Activity-based SPHK2 lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
SPHK2
Enzyme
Human SPHK2
HGNC
SPHK2
Synonym
Sphingosine kinase 2
Substrates
Sphingosine, 50 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Sphingosine + ATP = Sphingosine 1-phosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PF-543 | 36,000 |
Scientific Information
Q9NRA0 (http://www.uniprot.org/uniprot/Q9NRA0)
Screening Location
Malvern, USA
Reference compound IC50 for SPHK2
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