Activity-based SRMS kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SRMS
Enzyme
Human SRMS
HGNC
SRMS
Synonym
C20orf148, SRM, dJ697K14.1
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 5,300 |
| PP2 | 3,500 |
| AG1478 | 53,000 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 33,000 |
Scientific Information
Q9H3Y6 (http://www.uniprot.org/uniprot/Q9H3Y6)
Screening Location
Malvern, USA
Reference compound IC50 for SRMS
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