Activity-based STK16 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
STK16
Enzyme
Human STK16
HGNC
STK16
Synonym
TSF1
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 770 |
| Ro 31-8220 | 7,000 |
| GW 5074 | 42,000 |
| Rottlerin | 175,000 |
Scientific Information
O75716 (http://www.uniprot.org/uniprot/O75716)
Screening Location
Malvern, USA
Reference compound IC50 for STK16 10uM
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