Activity-based STK21/CIT kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
STK21/CIT
Enzyme
Human STK21/CIT
HGNC
CIT
Synonym
CRIK, STK21/CIT
Substrates
Peptide substrate, [KKRPQRRYSNVF], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 91 |
| Ro 31-8220 | 8,000 |
| GW5074 | 1,400 |
| H-89 | 36,000 |
| Rottlerin | >100,000 |
Scientific Information
O14578 (http://www.uniprot.org/uniprot/O14578)
Screening Location
Malvern, USA
Reference compound IC50 for STK21/CIT
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