Activity-based STK33 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
STK33
Enzyme
Human STK33
HGNC
STK33
Synonym
STK33
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 7.2 |
| Ro 31-8220 | 190 |
| GW5074 | 6,000 |
| H-89 | 2,800 |
| Rottlerin | >20,000 |
Scientific Information
Q9BYT3 (http://www.uniprot.org/uniprot/Q9BYT3)
Screening Location
Malvern, USA
Reference compound IC50 for STK33
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