Activity-based STK35/CLIK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
STK35/CLIK1
Enzyme
Human STK35/CLIK1
HGNC
STK35
Synonym
Serine/threonine-protein kinase 35, CLP-36-interacting kinase 1, PDLIM1-interacting kinase 1, PDIK1, STK35L1
Scientific Information
Q8WU08 (http://www.uniprot.org/uniprot/Q8WU08)
Assay Properties
Substrates
Peptide substrate, [GRSRSRSRSRSRSRSR], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 25 |
| Ro 31-8220 | 10,500 |
| GW5074 | 12,000 |
| H-89 | 28,000 |
| Rottlerin | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for STK35/CLIK1
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