Activity-based STK38/NDR1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
STK38/NDR1
Enzyme
Human STK38/NDR1
HGNC
STK38
Synonym
NDR1
Substrates
Peptide substrate, [KKRNRRLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.9 |
| Ro 31-8220 | 297 |
| GW 5074 | 7,100 |
| H-89 | 140 |
| Rottlerin | >100,000 |
Scientific Information
Q15208 (http://www.uniprot.org/uniprot/Q15208)
Screening Location
Malvern, USA
Reference compound IC50 for STK38/NDR1
PDF