Activity-based STK38L/NDR2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
STK38L/NDR2
Enzyme
Human STK38L/NDR2
HGNC
STK38L
Synonym
NDR2
Substrates
Peptide substrate, [KKRNRRLSVA], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.4 |
| Ro 31-8220 | 1,750 |
| GW 5074 | 22,000 |
| H-89 | 810 |
| Rottlerin | >50,000 |
Scientific Information
Q9Y2H1 (http://www.uniprot.org/uniprot/Q9Y2H1)
Screening Location
Malvern, USA
Reference compound IC50 for STK38L/NDR2
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