Activity-based SUV420H1-tv2 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SUV420H1-tv2
Enzyme
Human SUV420H1-transcription variant 2
Synonym
Histone-lysine N-methyltransferase SUV420H1, Lysine N-methyltransferase 5B Suppressor of variegation 4-20 homolog 1, KMT5B
Substrates
Oligonucleosomes, 0.05 mg/ml
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 5,400 |
| Sinefungin | 130,000 |
| BIX 01294 | >200,000 |
| Chaetocin | 33,000 |
Scientific Information
Q4FZB7 (http://www.uniprot.org/uniprot/Q4FZB7)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for SUV420H1-tv2
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